Neurocompare

Example.1In our lab, we use Chemicon International’s Rabbit Anti DFF 40/CAD Polyclonal Antibody (catalog #AB 16926) to confirm the presence or absence of CAD in the cytosol and nucleus of AMPA insulted pyramidal neurons using laser scanning confocal microscopy. We use Chemicon’s CAD Antibody to perform immunohistochemistry on hippocampal tissues of 8-15 day old Sprague-Dawley rats. We have been performing a temporal assessment of the presence or absence of CAD after the AMPA insult in both the CA1 and CA3 regions of the hippocampus.

This Anti DFF 40/CAD Antibody crossreacts with human, rat, and mouse tissue. The antibody is recommended for use in Western blots at a concentration of 1:500 – 1:1000. After performing a dilution curve we found 1:250 to be an optimal concentration for our immunohistochemistry studies. We detect CAD binding using a goat anti rabbit Alexa 488 secondary antibody from Molecular Probes for our fluorescent confocal microscopy analysis.

We have been using Chemicon International’s Rabbit Anti DFF 40/CAD Polyclonal Antibody for over three years now and have been extremely pleased with it. It has given us relatively consistent results. We have found Chemicon’s Rabbit Anti CAD Antibody to give a relatively strong signal in the hippocampal CA1 and CA3 pyramidal neurons. We chose this CAD antibody because it was the one we found that cross reacts with our species of interest (rats). Most others only cross reacted with human and sometimes mice. This is a relatively inexpensive antibody compared to many other antibodies for apoptosis studies. It also can be stored for up to 12 months in a standard 4^oC refrigerator, as opposed to other antibodies which are only good for 3 – 6 months and/or must be stored at -20^oC. I would definitely recommend using Chemicon International’s Rabbit Anti DFF 40/CAD Polyclonal Antibody to anyone who is doing apoptotic studies.

VelvetLee Finckbone, MS
Lab Technician
Texas Tech University Health Science Center

Example.2

I am trying to grow Caco-2 cells for intestinal permeability assays. The cells take about 3 weekes to grow and it is very difficult to obtain a uniform monolayer, most of the time they grow in clumps. I also see lots of cell debris in the flask. Is there a way to grow these cells better

Introducing Neurocompare

Neurocompare allows you to discuss your experiences with your fellows in this profession when you use a product for your research which may include a reagent or a software. You might also get help in designing your experiments with the right products with the experiences shared by other members.

Your suggestions are always welcome…